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2) The results of the assay in Figure 3 need to be quantified and it should be done by an objective measure using image analysis tools.
What is essential here is that the assessment be done by an objective third party who knows the composition of the reference sample that is unknown to the experimenter.
These data as well as a comparative quantification of the extent of overlap of ERGIC and mitochondria in cells transfected with Mit-TEER or cells with Mit-CC-TMCC3 is shown in Figure 5. 2) The results of the assay in Figure 3 need to be quantified and it should be done by an objective measure using image analysis tools.
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Couple that with the sheriff's announcement that they expect a search by Ground Penetrating Radar to last only a day -- a very limited time window -- it's important that analysis is done by an expert and objective authority.
This can be done by using an objective screening procedure that detects the weak and strong cognitive skills in brain function, thus helping design a tailored meditation training protocol.
This was done by an iterative optimization procedure minimizing the objective function stated in Eq. (19).
Focussing into the sample was done by a 1.3-NA oil immersion objective (Plan-Neofluar, Carl Zeiss AG) or a 0.85-NA water immersion objective (C-Achroplan, Carl Zeiss AG).
This will be done by globally minimizing an objective function which measures the quality of the fit.
The search for a local extrema θ∗can be done by iteratively evaluating the objective function and/or its derivatives at a current estimate θ(k) and by generating a sequence θ(1),θ(2),…y,θ(k) that converges to θ∗.
Further modifications of the FCM algorithm may be done by introducing more terms to the objective function (1), for example, in order to cope with noisy images [ 44].
However, if one wants to determine the breakpoint in an objective manner, this can be done by adjusting two linear regressions, one with the first q dots and another with the remaining dots, thus identifying the breakpoint (the value q) that minimizes the sum of squared errors.
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