In GISH experiments, the parental chromosomes could not be clearly distinguished, even when an addition of 50× unlabelled P. micranthum blocking DNA over the probe was added to the hybridization mixture (Fig. 2A C).
As shown in dilution experiments (Supplementary Figure 2), dissociation temperatures for partially methylated DNA can be distinguished from unmethylated DNA even when just a fraction (10%) of the cells show methylation.
Our data demonstrate that in individual children, changes in diastolic wall thickness or LVMI as a result of ESRD which are expected over a period of a year cannot be distinguished from measurement error, even when measured by the same observer.
Thus, the notion of gene essentiality has to be distinguished from the notion of lethality even when the environmental conditions are kept unchanged.
Changes in diastolic wall thickness of less than 1.6 mm or LVMI less than 17.7 g/m cannot be distinguished from measurement error in individual children, even when measured by the same observer.
However, because these words and picture-specific neuronal populations lie in close proximity to one another, they cannot be distinguished by functional magnetic resonance imaging (fMRI) (even when the spatial resolution is maximized, e.g., <2 mm as in Wright et al. 2008).
Even when measurements are perfect, structural isomers cannot be distinguished by mass alone.
Even small contaminant effects can be distinguished from background mortality.
As a result, the SHG signal can be distinguished from the fluorescence, and, in essence, the collagen can be imaged independently when even multiple fluorescent probes are used.
The sense of occasion is at its strongest when the cast is distinguished, but even unknown players in obscure performances can create it.
