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Cannabinoid containing resins can be dissolved by adding an emulsifier, a chemical that allows the mixing of oils and water.
If desired, the lipid bilayer can be dissolved by adding detergents.
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A dark brown color mixture was obtained, and it was dissolved by adding oxalic acid.
Following incubation, the insoluble formazan crystals were dissolved by adding DMSO (100 μL).
White residue, appear in the solution, was dissolved by adding NaOH solution and finally, the clear solution was obtained.
Then, the metabolized product MTT formazan was dissolved by adding 200 μL of DMSO to each well.
A white precipitate was obtained, and it was dissolved by adding oxalic acid (0.4 g in 30 mL DDW, 0.1 mole).
The formazan crystals that formed were dissolved by adding dimethyl sulfoxide (100 μl/well), and the absorbance was read at 570 nm using a microplate scanning spectrophotometer (ELISA reader, Organon Teknika, Netherlands).
The ash was dissolved by adding 8 mL of 100 mM HCl and analyzed for P concentration spectrophotometrically according to the Murphy and Riley method (Murphy and Riley 1962).
The bottles were transferred to the laboratory where the precipitate of the sample was dissolved by adding 1 ml of H2SO4, and 50 ml of this solution was titrated against 0.01 N sodium thiosulphate using starch as an indicator.
Any formed formazan crystals were dissolved by adding acidified isopropanol solution, and the absorbance of the solution was spectrophotometrically measured at a wavelength of 570 nm.
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