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Islets can be dissociated into single cells and reaggregated into so called "pseudoislets," which are functionally equivalent to intact islets but exhibit reduced immunogenicity.
The primary aggregation process causes formation of smaller sizes (∼103 nm) rod-like aggregation particles, which can be dissociated into single molecular chains by dissolving Nafion in propanol/water mixture solvents, is attributed to the hydrophobic interaction of fluorocarbon backbone.
Besides, our colospheres are very compact structures that cannot be dispersed by pipetting but require enzymatic treatment to be dissociated into single cells.
Similar(57)
(c) Importantly, we show that (α2ββ'ω 2 complexes can be dissociated into single-copy (α2ββ'ω) complexes by known protein interactors of the core enzyme, such as RapA, NusA, or σ70.
Briefly, cortical fragments were dissociated into single cells in dissociation solution (Sumitomo Bakelite, Akita, Japan) and resuspended in nerve culture medium (Sumitomo Bakelite).
Late gastrula stage embryos were dissociated into single cells by a washing once in dissociation buffer (1 M glycine pH 8, 2 mM EDTA) and then three times in calcium-free ASW.
Tumour biopsies were dissociated into single cell suspensions with collagenase digestion and/or mechanical dissociation.
Seven GBM biopsies from newly diagnosed, untreated patients were dissociated into single cell suspensions with papain at 37°C using the Neuronal Dissociation Kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) according to the manufacturer's protocol.
iNANOG cells and WTSG cells cultured with or without Dox, in either the E8 or 2iL/FK medium, were dissociated into single cells.
Luteal slices from mid-cycle corpus luteum were dissociated into single cell suspension in aerated (O2) culture media (DMEM/F12) by enzymatic digestion.
EBs were dissociated into single cell suspensions after trypsinization.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com