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Differentiation of genetically corrected patient-specific iPS cells [45] might ultimately produce NSC grown as neurospheres that could be dissociated for transplantation to generate both neurons and their supportive glial cells.
It seems equally possible that SPB-kinetochore connections must be dissociated for normal execution of prophase I events, e.g. telomere-nuclear envelope attachment, chromosome movement, etc. 5.
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Day 3.5 EBs were dissociated for downstream functional assays or gene expression analysis.
"I was dissociated for many years".
Cells were dissociated for 5 hours by gentle shaking.
Colonies were dissociated for passaging using trypsin with 3 mm glass beads.
The antigen-antibody complexes were dissociated for 30 minutes at 37°C and the reaction was stopped by addition of 1 volume of neutralization buffer (1.5 M Tris-HCL [pH 9.7]) to achieve an end dilution of 1∶3 for the sample.
EBs were dissociated for flow cytometric analysis or CD34+ cell enrichment by FACS by the addition of 1 mg/mL collagenase B (Roche) for 2 hours at 37°C, following by vigorous pipetting.
Mammary glands were dissected and minced with scissors, then the cells were dissociated for 8 h at 37°C in EpiCult-B with 5% fetal bovine serum, 300 units/ml collagenase, and 100 units/ml hyaluronidase.
Carcasses were washed twice in PBS, minced finely, pieces were dissociated for 10 min with rotation at 37°C using trypsin/EDTA solution, triturated and filtered through a 70 μm mesh.
One hour before surgery, hNPCctx-GDNF or hNPCctx neurospheres were dissociated for 10 minutes in Accutase (1 millionillion cells) followed by inactivation with an equal volume of 0.2% trypsin inhibitor.
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