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We show that changes in LFP gamma power and spiking activity in the primary visual cortex (V1) of the awake primate can be dissociated by using grating and plaid pattern stimuli, which differentially engage surround suppression and cross-orientation inhibition/facilitation within and between cortical columns.
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Salt can be dissociated by the interaction between salt anions and surface OH groups of Al2O3.
The acid-soluble constituents can be dissociated by acidification after dissolution in alkaline medium [13].
Then, the ions were dissociated by ECD using 3 4% "electron energy" and a 70 ms duration with a 65 μs delay.
After washing with fresh Neurobasal medium (containing B27 supplement, Gibco, Life Technologies), ganglia were dissociated by trituration using a fire-polished Pasteur pipet.
The neurons were dissociated by gentle pipetting using a 1,000 µl micropipette tip, and the resultant cell suspension was placed onto poly-D-lysine (Sigma-Aldrich Japan, Tokyo, Japan -coated coverslips which were placed in a 35-mm diameter culture dish and left for 60 min. The B. mori neurons were then incubated at 25°C for 18–36 h before electrophysiology.
Tissue was dissociated by shear forces using a 10 mL pipette and filtered through a tube-top cell strainer (pore size, 100 μm; BD Biosciences, San Jose, CA, USA).
Cells were dissociated by gentle pipetting and collected using centrifugation.
We have previously shown that yeast enolase is dissociated by NaClO4; under the same concentrations used in these experiments, complete dissociation of the yeast enzyme requires 0.3 M NaClO4 [19].
Cells were dissociated by repeated trituration, collected by centrifugation, counted using ViCell (Beckman Coulter, Miami, FL) and diluted to at least 10 cells per 50 µl of PGB.
Cross-links were dissociated by heating at 65°C overnight, and DNA purified using Qiaquick (QIAGEN).
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