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Furthermore, we show that molecular mechanisms of complex tissue regeneration can now be dissected using zebrafish genetics.
Aa-tRNAs are delivered to and selected by the ribosome via a multi-step process (Rodnina and Wintermeyer, 2001), which can be dissected using smFRET between P-site tRNA and incoming aa-tRNA at high data collecting rate (Geggier et al., 2010).
When expression levels were compared among mice of different genotypes, lack of TNFα mRNA was confirmed in TNFα−/− and ApoE−/−/TNFα−/− animals, but no significant differences in the basal levels of the other studied targets could be dissected using RNA from whole retinas (Figure 4).
However, the carcinogenic mechanism of air pollution remains to be dissected using systematic approaches.
Furthermore, the stem cell population identified using the ALDEFLUOR assay is probably heterogeneous, and needs to be dissected using additional markers.
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Prostates from transgenic mice were dissected using a dissection microscope, fixed in 10% buffered formalin and embedded in paraffin.
Following 48 h of fasting, mice were euthanized and soleus, plantaris, gastrocnemius, tibialis, and extensor digitorum longus were dissected using standardized dissection methods.
The CA1 region was micro-dissected from dorsal rat hippocampus or whole hippocampus was dissected using pre-chilled RNase free instruments under a light microscope.
The intracellular mechanisms involved were dissected using inhibitors of intracellular mitogen-activated protein kinase (MAPK) activity.
Adhered particles were dissected using a dual-beam scanning electron microscope/focused ion beam (SEM/FIB) for morphological and microstructural examination.
Prefrontal cortex (PFC) and hypothalamus (HYP, excluding SCN) regions were dissected using a brain slicer matrix (Zivic Instruments, Pittsburgh PA USA) and they were immediately frozen in dry ice.
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