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Based on the different levels of sensitivity to inhibition, Pol II engagement can be dissected into at least two sections: the release of the downstream elements from TFIID (which can partially occur in the presence of the inhibitor) and the binding and reorganization of the TATA-Inr region (which is almost completely inhibited).
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The proposed PseB reaction mechanism can be dissected into three distinct steps starting with oxidation at the C4 position of UDP-GlcNAc through a catalytic base (Y141) and reduction of NADP+.
The remaining loci were dissected into groups at increasing stringency levels of the linkage test (LOD values from 3 to 12).
Chicken embryos were dissected into PBS at 8 days of incubation, avoiding contamination with yolk, heads were removed and placed into 4% PFA, 2.5% glutaldehdye in 0.1 M cacodylate buffer.
As an example shown in Fig. 1h, the molecule is dissected into two parts at the bond linked by nitrogen and carbon.
Salivary glands were dissected into 4% paraformaldehyde and fixed for at least 30 minutes at room temperature.
Briefly, the ILT was dissected into three parts [28]: luminal (at the interface with the circulating blood), intermediate, and abluminal layers.
Epiphyseal regions were dissected into 1 mm segments immediately at sacrifice while immersed in 2.5% glutaraldehyde in a cold environment.
The brain is dissected into 528 coronal tissue sections at 25 μm separation, which are Nissl-stained, registered to each other, and assembled into a reconstructed brain volume.
Replicate leaves were dissected into laminal sectors and frozen in liquid N2 at 12 or 24 h after the time of 1st elicitation.
Immature grain at 12 days post-anthesis were dissected into endosperm, inner and outer seed coat/pericarp tissues and RNA extracted as described by Wan et al. [ 69].
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