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As noted, the ECM or outermost layer must be disrupted to extract and recover biomolecules.
The plant tissue must therefore be disrupted to extract the product, challenging the initial DSP steps with an unusually high load of both particulate and soluble contaminants.
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Cells were mechanically disrupted to extract intracellular peptides.
Its business began to be disrupted.
This balance was soon to be disrupted.
Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood using BD Vacutainer CPT Cell Preparation Tubes (Becton, Dickinson and Company, Franklin Lakes, New Jersy), and cells were disrupted and RNA extracted with TRIzol Reagent (Invitrogen, Carlsbad, California).
Disrupt or be disrupted.
Will he disrupt or be disrupted?
Algal cells were disrupted and DNA was extracted with MO BIO PowerPlant DNA Isolation Kit (MO BIO Laboratories, Carlsbad, California, USA) according to the manufacturer's protocol.
To obtain total protein extracts, mature pollen was disrupted using extraction buffer containing detergent (0.5% NP-40).
The cells were disrupted by vortex and extracted at 4 °C for 30 min.
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