First, the size of deletions analyzed by Margot et al. [12] were large, and because of this small regions of DNMT1 that are dispensable for function were likely not identified.
Hence, the Pab1 interacting motif PAM2 was dispensable for function.
Taken together, the PAM2 motif is dispensable for function of Upa1.
A C-terminal coiled-coil has also been identified in Cdc11, but it is dispensable for function.
Phenotypic analysis revealed that the RING domain (Upa1ΔR-Gfp) was dispensable for function under the tested conditions, whereas deletion of a C-terminal region containing the RING and FYVE domain (Upa1ΔFRGfp) results in loss of function.
In the C1C2 X-ray structure, Asp253 (ChR2 numbering) is in close proximity with the RSBH+ compared to Glu123, and the electrical studies on various ChR2 mutants revealed that Glu123 (but not Asp253) is dispensable for function.
Deletion of the C-terminal lectin-binding (β-prism-like) domain of RbmC, that is absent from Bap1, indicates that this domain is dispensable for function (Absalon, Van Dellen and Watnick 2011).
On the other hand, some ribosomal proteins (e.g., S16, L15, L16, L20, and L24) are mostly essential for the assembly of the RNP particle and are dispensable for function after the ribosomal subunits are fully assembled, suggesting that their major function in the assembled ribosome is related to the improvement of the ribosome stability.
On the basis of the results, they proposed that Oct-4 is dispensable for functions of somatic cells.
This part of US2 has also been shown to be dispensable for US2 function.
