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ZFN-encoding RNA can be directly transcribed from these plasmids using T7 RNA polymerase.
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Since RNA probes were directly transcribed from human genomic sequence, it was necessary to test the probes for repetitive sequences.
Different from this siRNA model, the biogenesis of intergenic miRNA, eg, lin-4 and let-7, involves a long noncoding precursor RNA transcript (pri-miRNA), which is directly transcribed from a Pol-II or Pol-III RNA promoter, whereas intronic miRNA is co-transcribed with its encoding gene by only Pol-II and released after RNA splicing as a spliced intron.
Next we focused on lncRNAs that are directly transcribed from SEs, as SEs were much more likely to have lncRNA transcription than CEs (8.3 % of SEs vs. 0.9%% of CEs in Th17 cells, p-value < 2.2e-16 2.2e-16n's Chi-squared test) (Fig. 3d).
The ease to synthesize them (they are directly transcribed from DNA) and the broad diversity of catalytic and regulation functions they can perform enable to integrate de novo logic circuits within living cells (Rodrigo et al., 2012) or reprogram existing regulation mechanisms (Chang et al., 2012).
The structure of the model can be directly transcribed to a process control computer to mimic plant operation.
Using a high-strength metal stamp, nano structures were directly transcribed to a ductile metal film or curved/3D surfaces.
The collected cells were directly transcribed using TaqMan Gene Expression Cells-TaqMan Gene Expression
Much that was written was either directly transcribed from the earlier medieval chronicles themselves or was modelled closely upon them, even in the significant works of John of Fordun, Andrew Wyntoun and Walter Bower.
The relevant relations for these real-valued spherical harmonics are best directly transcribed from the corresponding complex relations.
Ligated samples were directly reverse transcribed using SuperScriptIII (Invitrogen), circularized using CircLigase (Epicenter), and PCR amplified.
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