Reactions were performed at 30°C for 30 min and then stopped at 75°C for 10 min. Subsequently, reaction products were directly separated on a 1% agarose gel and stained with ethidium bromaide (1 µg/ml) for 20 min. A 1.4 Kb linear attR fragments band densities were analyzed by using a UVP-GDS8000 gel analysis system Ultra-Violet Products Ltd , Cambridge, UK).
Beads after washing were directly separated on 10% SDS-PAGE and then transferred to Hybond™-C extra membrane (Amersham).
Alternatively, on native PAGE, such PCR products can be directly separated due to the differences in configuration and length.
The second amplification products from eba-175 and msp-3 were directly separated by electrophoresis on a 2.0% ethidium bromide agarose gel and visualized on a GEL LOGIC 220 UV-Transillumination Imaging System (Carestream, Rochester, NY).
Amplicons were either directly separated on a 2% agarose gel (InDels) or first digested by a SNP specific restriction enzyme (as indicated in the marker name in Table 1) according to the manufacturer's protocol.
Based on the meso-scale structure, the network may be decomposed into small, separate sub-networks which can be directly re-engineered with significantly lower complexity.
To clinicians who focused on treating TB, reducing smoking was considered as a separate issue, not shown to be directly beneficial.
At specified time points, samples were removed and protein directly separated by SDS-PAGE.
Firstly, large quantities of 4Cv product were separated on a gel and the bands were excised, re-amplified, and sequenced directly.
