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This finding suggests that the ribose moiety may be directly recognized by amino acids from hENT1.
In lower eukaryotes, introns are generally small and could be directly recognized by the splicing machinery the intron definition model; but in higher eukaryotes, such as vertebrates where most of introns have been expanded, the splicing machinery would use exons as the unit of recognition to facilitate the identification of exons among the intronic oceans the exon definition model [27], [28].
In "classical" nuclear import, importin-α recognizes and binds the target proteins in the cytoplasm, mediating their transport across the nuclear pore complex after formation of a ternary complex with importin-β [48], although some NLS-containing cargoes can be directly recognized by importin-β without the need of the adaptor protein.
In these cases the stem is often essential to the attainment of the correct secondary structure and may be directly recognized by ribonucleases [ 20- 23].
Alternatively, the PAH molecule may be directly recognized by a receptor such as a PAS-domain protein, a large and widely-distributed class of environmental sensors that includes the vertebrate aryl hydrocarbon receptor [ 46, 47].
This repressive chromatin mark can be directly recognized by the N-terminal chromodomain of chromobox proteins (CBX2,4,6,7,8) [ 24], which then recruit PRC1 members to chromatin via a C-terminal domain [ 25].
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Only when the sequence corresponding to a given miRNA binding site was directly recognized by a probe-set or was located upstream to the sequences detected by the probe-set in the gene, did we consider this probe-set to be affected by the activity of this miRNA seed.
We used the following rule for assigning probe-sets to miR-seeds: when the sequence corresponding to a given miR-seed is directly recognized by a probe-set or is located upstream to the sequences detected by the probe-set in the gene and on the same exon, we consider this probe-set to be a reliable indicator of activity for this miR-seed.
Lipid A is directly recognized by hosts to trigger innate-immune responses [ 45, 46].
Therefore, we propose a model in which helix 5 and the 3′ overhang are directly recognized by Exportin-5.
(It should also be noted that the promoter elements of Pol III-transcribed genes are directly recognized by TFIIIC and not by Pol III/IIIB).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com