Finally, after extensive washing, the immunolabeled brain samples were directly cleared in FocusClear (CelExplorer, Taiwan) for 5 min and then mounted in a drop of MountClear (CelExplorer) and then imaged under a Zeiss LSM 510 confocal microscope.
At the three nuclear genes, almost all samples of A. aureum and A. speciosum, and most samples of the putative hybrid, could be directly sequenced and clear sequences were obtained.
In parallel, Deisseroth and colleagues have developed a technique called STARmap, in which up to 1,020 different genes can be directly sequenced within cleared brains9.
Like other Extensional accounts, it allows change and succession to be directly experienced in a clear and unambiguous manner, and so fully satisfies the requirements of realism.
On Niue, most forest clearing can be directly attributed to subsistence gardening activities.
When D-NT and N-NT miRNA levels were directly compared, clear differences were observed: 200 miRNAs were significantly dysregulated, with 116 up-regulated and 84 down-regulated.
Although the functions in (19) can be calculated numerically, it is not directly clear how σ2 (E i,Δ t j ) is related to the parameters σ o, j 2, α j, β ij and Δ t j.
Although for histopathological quantal data the deterministic interpretation is obvious from first principles, it is not directly clear whether cancer or malformation quantal data should be treated as stochastic or as deterministic quantal data (Slob et al. 2014).
While it's not directly clear why a financial trading site would be interested in acquiring a cross-platform extensions developer, it may be a case of an aqui-hire for developer talent, or perhaps for other parts of Markets.com's business — it is owned by entrepreneur Teddy Sagi's Safecap Investments.
Furthermore, in a multi-user oriented system, such as LTE, it is not directly clear which figures of merit should be used to assess the performance of the system.
