Sentence examples for be digested into single from inspiring English sources

Importantly, the pips_m cells demonstrated good cell viability and could be digested into single cells and passaged easily.

Two days after virus infection, infected MEFs were digested into single cell and seeded at 5,000 cells per well into 96-well plates pre-seeded with irradiated MEFs or pre-coated with Matrigel (BD biosciences, Cat. No. 356234).

In order to understand the influence of 3D culture on NT, MEFs cultured in 3D collagen scaffolds were digested into single cells and served as donors for NT (Fig. 2D).

To obtain single cell-derived clone, cells are digested into single cells, loaded through 40-μm Nylon mesh and seeded with extremely low density, then a single colony grown up from a single cell was picked up by clone cylinder (Sigma, USA) and high vacuum grease after its neighboring colonies were cleared by scraper to ensure the pedigree purity.

Harvested brain tissue samples were digested into single cell suspension using papain as described in the methods.

The scaffolds were digested into single cell suspensions using collagenase type II (Worthingtion, 250 U/ml).

Mouse ES or human ES differentiated cells at terminal differentiation day were digested into single cells with Accutase.

Cells in logarithmic growth phase were digested into single cell suspensions, which were diluted and transferred into 25 mL culture flasks with 5 × 10 cells per flask.

H9 cells were digested into single cells by accutase and plated in 6-well-plates at a density of 1 × 10 cells/well in mTeSR1 medium.

After that, neurospheres were digested into single cells by accutase (STEMCELL Technologies), which can either be maintained as NPCs in NPC medium or further be induced to neurons or glia.

Moreover, the expressions of cancer stem cells markers, such as Oct4, Sox2 and Nanog were gradually decreased when the sphere cells were digested into single cells for re-adherent culture.