Upon the addition of Exo III, the dsDNA templates would be digested from 3′ to 5′, and the formation of fluorescent Cu NPs would be inhibited.
Glucose and amino acids can be digested from starch and protein in small intestine, respectively.
The rabbit Axin probe was digested from pcDNA3-FLAG/rAxin (full-length) (gift from Dr A Kikuchi, Hiroshima University, Hiroshima, Japan).
The mGluR1 Lc fragment was digested from pCAGGS-mGluR1Lc construct (a gift from Dr. Michisuke Yuzaki) and subcloned into the pUAST vector.
Unlike rumination, gastroparesis causes vomiting (in contrast to regurgitation) of food, which is not being digested further, from the stomach.
To construct pIRES-hyg-pcDNA3.1z, IRES (internal ribosome entry site from virus EMCV) was digested from pBL-EP (kindly provided by Dr. John Olsen, University of North Carolina) by EcoR I and EcoR V and cloned into linearized pcDNA3.1z vector.
Cloned DNAs used as probes were digested from their vectors or PCR amplified, electrophoresed, and purified from agarose using the QIAquick gel extraction kit (QIAGEN, Valencia, CA).
For the mir-34 promoter, the 5-kb promoter was digested from a separate plasmid and ligated to a modified 476p5Emcs (a gift from Nathan Lawson's lab, modified to remove unnecessary SalI cut sites) to add the necessary att sites.
Primary osteoblasts were digested from 6- to 8-day-old mouse calvaria.
In addition, more information is digested from the Pareto optimum solution set by cluster analysis and fuzzy set theory.
