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Arabidopsis root +Pi vs − Pi DMRs were identified (FDR < 0.2) between 10 days +Pi and 10 days −Pi samples, requiring 3 of 3 −Pi root samples to be differentially methylated compared to +Pi root samples.
Rice shoot +Pi vs − Pi DMRs were identified (FDR < 0.05) between 21 days +Pi and 21 days −Pi samples, requiring 3 of 3 −Pi shoot samples to be differentially methylated compared to +Pi shoot samples.
Rice root +Pi vs − Pi DMRs were identified (FDR < 0.01) between (21, 22, 24 days) +Pi and (21, 22, 24 days) −Pi samples, requiring ≥7 of 9 root −Pi samples to be differentially methylated compared to root + Pi samples, as well as enabling 1 of 9 root −Pi samples to be similar to + Pi and vice versa.
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This conclusion is similar to that drawn from other studies which found that both the bodies and promoters of highly expressed genes are differentially methylated compared with inactive genes [ 30, 31, 38, 50].
Only HOXA9 was found to be differentially methylated in 3q8pq20 compared to non-3q8pq20 tumors, as well as between node positive and node negative cases.
Of the CNL-enriched probes, 11.3 % were significantly differentially methylated, compared to 21.7%% of CNN-enriched probes (p < 0.0001, Chi-squared test, Fig. 2c).
Forty genes have been shown to be differentially methylated in PCOS patients compared with the corresponding genes in normal individuals.
Therefore, the regions that were identified to be differentially methylated in the LNCaP compared to PrEC cells as identified in this study by our overall MBD-chip and associated computational approaches are likely to be highly enriched for effective DNA methylation biomarkers for prostate cancer identification.
Genes found to be differentially methylated without butyrate addition (compare with Fig. 3b, c) were removed from the '24 h' and '48 h' datasets before cluster analysis.
We observed a total of 14,742 DMRs in the seven NSCLC tumor samples (Additional file 9), that is regions found to be differentially methylated in at least one tumor when compared with its lung counterpart with a two-fold change difference in methylation signal.
However, other studies have identified significant differences in the KCNQ1 gene, which we found to be differentially methylated in NFF vs. DFUF contrast, when comparing diabetic to non-diabetic subjects in pancreatic β cells, in adipose tissue of MZ twins discordant for type 2 diabetes, and in placenta and cord blood in mothers with gestational diabetes.
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