Probes from tumour tissue and from the reference tissue were differentially labeled by the incorporation of cyanine 3 (Cy3) and cyanine 5 (Cy5) (Dual Color 44K microarray, Agilent Technologies), respectively.
Comparing these 32 features with the 95 identified as being differentially labeled by XCMS, we discovered that only two were recovered by both analyses.
The probes are from tumor tissue and from reference tissue that are differentially labeled by the incorporation of cyanine 3 (Cy3) and cyanine 5 (Cy5), respectively.
Most work on the kin conflict theory of imprinting has focused on how maternally and paternally derived genes are selected, assuming they have been differentially labelled by the parents.
To study the neuroanatomical features of the two lineages in the mature brain, we took advantage of the fact that they are differentially labelled by four enhancer-Gal4 driver lines in the adult brain.
In brief, 2 μg of test DNA and 2 μg of reference DNA were differentially labelled by random priming using Cy3-dCTP (PA53021, GE Healthcare, Piscataway, NJ) and Cy5-dCTP (PA55021, GE Healthcare).
In a common microarray hybridization, two pools of RNA (e.g. from control and treated cells) are differentially labeled (usually by Cy3- and Cy5-labeled nucleotides) and co-hybridized to a glass slide having either PCR products ("cDNA array") or gene-specific oligonucleotides ("oligo array") covalently attached to its surface.
After digestion, fragments longer than approximately 80 bp and shorter than approximately 2.5 kb are isolated by sucrose-gradient fractionation, and test and reference DNAs are differentially labeled with fluorochromes by random priming.
The probes were differentially labeled and visualized by confocal microscopy.
Here we would also see spatial patterns in a biofilm, but I doubt that one would find the enhanced population mixing (given that two biofilm-formers were differentially labeled) as shown by the authors.
