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Since genes with similar functions tend to have similar expression patterns [15] [17], we performed hierarchical cluster analysis on all genes found to be differentially expressed based on their expression relative to the mixed blood stage.
In this study, we found that none of the toxin cluster genes was identified to be differentially expressed based on the 2-fold difference in expression level and P value less than 0.0001 criteria.
There are 252 transcription factors in the right-tail of the primitive erythroid score distribution, of which 144 were found to be differentially expressed, based on ranked cosine similarity, between the adult definitive and primitive erythroid expression datasets.
While the RNA produced might itself be an input to a downstream device, more classically genes may be placed to the left and right of the device and be differentially expressed based on whether the sequence A and then B occurred or the reverse.
Molecular pathways that were predicted to be differentially expressed based on computational analysis were validated by performing RT-qPCR on RNA extracted from diseased and non-diseased palmar fascia biopsies.
A total of 1,157 ESTs were found to be differentially expressed based on t-test, with a Benjamini and Hochberg false discovery rate for multiple testing correction at P ≤ 0.05.
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A total 587 genes (∼2% of the total genes profiled) were differentially expressed based on the set analysis criteria.
Following microarray analysis to determine the gene expression profiles of M059K and M059J cells, we found that a total of 587 genes (approximately 2% of the genes analysed) were differentially expressed based on the set criteria (p<0.05; fold difference of 2) as indicated in Figure 5B.
Consistent with the microarray results, the four lncRNAs were differentially expressed based on QPCR.
In A. salmonicida, it has been shown that a number of virulence factors were differentially expressed based on environmental conditions.
For instance, several isoforms of the L subunit are differentially expressed based on the type of tissue in the plant.
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