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We expected that the abasic-bypass reporter activity should be different in cells carrying different levels of FANCD2 protein expression, as suggested by data shown in Figure 3.
While, the adipogenic interference effect that we observe may be related to cell density, we should not ignore the fact that the expression of cellular adhesion proteins may be different in cells forming aggregates compared to cells adherent to substrates.
One possible explanation is that regulation of the IL10 gene may be different in cells of the innate and adaptive immune system, and genetic associations may consequently differ for various pathologies.
Following the latter consideration, we hypothesized that EC response to gravitational alteration could depend on the district from which the cell population derives and could be different in cells derived from macro- or microcirculation.
Indeed, the global gene expression changes induced by estrogen and anti-estrogens are known to be different in cells expressing both estrogen receptors relative to cells expressing only ERα or ERβ [ 20, 21, 56].
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Interestingly, intersperse repetitive sequence (IRS) hypomethylation patterns are different in cells with different phenotypes.
The situation was different in cells overexpressing S3A and S3D cofilin.
Note, however, that this rapid cell migration of myosin IIA deficient cells is unique to 2D cell migration, and the phenotypes are different in cell migration in a 3D environment (see Discussion).
Second, the SOX2 response program could be different in different cells (i.e. GBM vs. ES cells).
Since these studies use isolated mitochondria, the situation might also be different in intact cells and tissues.
Because different types of tumor cells have different genetic alterations and characteristics, the molecular targets of EGCG may be different in different cell lines.
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