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In addition, an FMIA could be developed for differentiation of wild-type infected vs vaccinated animals (DIVA) if proteins used in the vaccine were different from those produced in a wild-type infection.
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We believe our system not only provides a platform for producing therapeutic cells from hESCs for PD patients, but also a generalized plan for how hESC culture and lineage-specific differentiation need to be developed for treatment of other degenerative disorders, including spinal cord injury and type I diabetes.
Specific primers for C. minus simile and C. minus verum were developed for molecular differentiation from pure cultures and direct amplification from infected needles.
Further, two oligonucleotide probes targeting the 16S rDNA were developed for subspecies differentiation by a one base-pair difference following identification of the species.
Real-time TaqMan-PCR assays were developed for detection, differentiation and absolute quantification of the pathogenic subspecies of Clavibacter michiganensis (Cm) in one single PCR run.
A variety of different needle-sized DRS probes have been developed for tissue differentiation [ 9].
Several methods have been developed for genetic differentiation of other fish pathogenic bacteria [ 9, 10].
TaqMan probes (Roche, Indianapolis, IN, USA) were developed for rapid differentiation of MIDV and SINV by real-time PCR (online Technical Appendix).
To our knowledge, no phage typing system has been developed for the differentiation of Dickeya spp., and phage typing of Pectobacterium spp. has not been applied in ecological research.
To investigate the cellular and molecular mechanisms underlying haematopoiesis, serum-free culture systems were developed for unilineage differentiation and maturation of cord blood CD34+HPCs through the erythroid (E), megakaryocytic (MK), granulocytic (G) or monocytic (Mo) pathways.
SYBR Green I real-time PCR was developed for detection and differentiation of Newcastle disease virus (NDV).
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