Confidence limits (CLs) were determined for quantification of the degree of agreement that should be expected in each DQA method.
The DAB and haematoxylin channels were isolated using a plugin and a threshold was determined for quantification.
Then, green, red, and blue channels were isolated using ImageJ software, and a threshold was determined for quantification of signal intensity.
The PCR products were purified using Ampure beads (Beckman Coulter Inc., Brea, CA, USA) and the resulting libraries were determined for quantification and size profiling using Qubit and BioAnalyzer 1000 (Agilent Technologies, Santa Clara, CA, USA).
Standard deviation was determined for all quantification measures.
TUNEL staining was examined under fluorescence microscope (Olympus, Japan), for each group in given experiments, at least 300 randomly chosen cells were determined for the quantification.
Optimal detection conditions were determined for the histamine quantification by ELISA method.
For relative DNA quantification (and, by extension, mRNA), a quantification value was determined for each of the six genes studied as follows: quantification factor = 10W, in which w = [linear coefficient − (Ct × angular coefficient)].
Finally, the accuracy of the quantification was determined for the large uniform part of three different phantoms.
The limit of detection and quantification were determined for the three laboratory obtained fibres (MA, MMA and MA/MMA).
