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The recently introduced FloTrac/Vigileo™ system (Edwards Lifesciences, Irvine, CA, USA) on the other hand allows the cardiac output to be determined continuously using pulse wave analysis without external calibration.
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Gas exchange was determined continuously using a breath-by-breath portable measurement system (K4b2, COSMED, Rome, Italy).
In general, the concentration of gaseous pollutants and RSP are determined continuously by automatic analyzers.
The measurements of the angles were determined by using drawing tools in Impax DS3000 (AGFA), and registered continuously in a database.
ACE activity in aortae extracts was determined using Abz-FRK(Dnp)P-OH derivasivesubstratesrates by continuously measuring the fluorescence according to our previous publications [ 21, 22].
Heart rate was measured continuously by means of an electrocardiogram, and blood pressure was determined using a non-invasive method at 10 minute intervals at the left arm.
Peptide binding was determined using ELISA.
Iodine uptake was determined using radioactive iodine.
Signal intensity was determined using GeneChip Operating Software 1.4.
Insertion sites were determined using semirandom PCR [31].
Protein concentrations were determined using RC DC Protein Assay (BioRad).
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