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As anticipated, we also observed such an effect on the metabolome in the current study, suggesting that terroir-specific effects can only be determined by including multiple vintages in the analysis, to avoid confusing terroir-specific effects with differences caused by the growing season.
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The inhibitory effect of acetyl-CoA on the liver PanK activity was determined by including increasing concentrations of the compound in the reaction mixtures, as indicated.
To observe compaction, the TRF2-dependent change in effective radius (Re) of the nucleosomal array fibers was determined by including microspheres of a known radius in each multi-gel experiment and utilizing a sieving equation [47], [54], [55].
Background levels of hybridization were determined by including, as negative controls, 4308 randomly generated sequence oligo probes that did not correspond to any Tetrahymena genome sequence but were of comparable length and GC content to the experimental probes on each array.
Nonspecific binding was determined by including 1,000-fold 1,000-foldRy.
The background fluorescence of resazurin itself was determined by including a resazurin only control.
Inter-assay variation was determined by including duplicates of dilutions of the plasma pool on every plate measured.
The critical required ions are determined by including the most abundant ions contributing to 75% of the total isotope area.
For each gene, additional family members were determined by including genes that were listed as ambiguous orthologs (1 many) or by a separate Ensembl within species search for paralogs.
The reproducibility of the PCR assay was determined by including a standardisation sample in 17 consecutive PCR plates, run by 2 independent manipulators.
This was determined by including parent-reported negative childhood emotionality and recent life events retrospectively recalled by adolescents as potential modulators of any effects of childhood adversity on depressive symptoms within the adolescent years.
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