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Thus, the suitability of the cerebral cortex or central semiovale as a reference region has yet to be determined by blocking or occupancy studies.
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Specificity of antibody binding was determined by blocking with cognate peptide.
GLUT4 protein content was determined by blocking in TBST + 2% skim milk and primary antibody from Chemicon (AB1346).
Specificity of the TTP antibody was determined by blocking with the identical peptide sequence used to generate the polyclonal antibody.
HKII protein content was determined by blocking in TBST + 3% skim milk and primary antibody from Alpha Diagnostic international (San Antonio, TX, USA).
Cyt C protein content was determined by blocking in TBS with Tween (TBST) + 3% BSA and primary antibody from BD Biosciences (Pharmingen, San Diego, CA, USA).
Expression of FcγRIII was determined by blocking the cells with 5 μg/mL FcγRIIB-specific mAb (clone K391, Ly17.2-specific) followed by staining with anti-FcγRII/III (2.4G2, BD Pharmingen).
The effect of CD109 on ECM synthesis was determined by blocking CD109 expression using CD109-specific siRNA or addition of recombinant CD109 protein, and analyzing the expression of ECM components by western blot.
Treatment assignment among the two groups was determined by blocked randomization.
Treatment allocation was determined by blocked, stratified randomization with a 1 1 distribution to TIV or placebo; randomization was stratified by study center, age 18-344 and 35-49 years), and the subject's report of previous recent receipt (within ≤ 2 years) of TIV.
The order of infusion was determined by block randomisation.
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