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The major histocompatibility complex (MHC) binding motif can be determined by binding or functional assays using analogs of the minimal active sequence.
Therefore, a cytosine-5 methyltransferase with activity towards both DNA and RNA substrates could function in any compartment containing nucleic acids and the specificity to the respective substrate could be determined by binding partners.
In recent years, it was found that nucleus localization of AGO4 could be determined by binding to small RNAs [ 24].
The presence of cell-surface receptor protein on live cells can be determined by binding assays with labeled ligand or by flow cytometry with labeled antibodies to the receptor.
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The activity of RhoA, Rac1 and Cdc42 was determined by binding to GST-rhotekin-RBD, GST-PAK-PBD and GST-WASP-PBD respectively [50], [51].
Additionally, potential gene interactions were determined by binding site predictions.
Non-specific binding was determined by binding in the presence of 50-fold molar excess of cold ligand.
At and around the adhesions, the translational dynamics is determined by binding interactions with partners that are concentrated in these regions.
To induce a CTL response, the epitope-precursor fragments must first be transported by TAP into the ER, and, because TAP transport rate is determined by binding, not translocation (Gubler et al., 1998), we measured the normalized TAP affinities of the precursors.
Some fluorescent probes have specific binding to different regions of the HSA, and the binding sites can be determined by the displacement binding experiments using some probes.
This nuclear localization may be determined by the first RNA-binding stretch aa 76 109.
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