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The amount of BrdU in the DNA of cells can be detected with specific anti-BrdU fluorescent antibodies followed by flow cytometry.
Protein A could be detected with specific monoclonal mouse antibodies raised against Protein A. It was found that ecsA::intron mutation clearly decreased Protein A levels in the wall (about 3-fold as compared to the wild-type strain; Figure 2A).
This suggests that they could be detected with specific antibodies without the need to permeabilize cells.
In cells that express EpoR, however, <10%% of EpoR protein appears on the cell surface; the remainder is degraded into intracellular EpoR fragments that can be detected with specific EpoR antibodies [ 12, 21, 35, 36].
The natural course of a NBL tumour, although still uncertain, can be partially prognosticated: the biologic character of the tumour can be appreciated, its extent can be demonstrated with increased accuracy using current imaging modalities (I-MIBG, MRI, PET) and minimal disease can be detected with specific tests (immunocytology, reverse transcription-polymerase chain reaction).
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Microbiologists often identify bacteria by the presence of specific molecules on their cell surfaces, which are detected with specific antibodies.
Primary antibodies were detected with specific anti-rabbit or anti-mouse-IgG-HRP (Cell Signaling Technologies).
Subsequently, the LipA and TrxA proteins were detected with specific polyclonal antibodies raised in rabbits.
Proteins of interest were detected with specific antibodies, followed with infrared dye 700 or 800-conjugated secondary antibodies.
This time, however, RNA protein complexes were transferred to PVDF membranes and interacting proteins were detected with specific antibodies.
TRAIL, Flag, Sp1, and β-actin were detected with specific antibodies following blotting with a horseradish peroxidase-conjugated secondary antibody and visualized using an ECL detection system.
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