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Molecular interactions can be detected using single engineered ion channels as stochastic sensors.
Our results provide evidence for multiple genetic effects that may not be detected using single SNP analysis.
Failure to detect eQTL may be explained by multiple regulatory elements in which effects cannot be detected using single marker analysis.
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DNA strand breaks were detected using single cell gel electrophoresis/comet assay, using standard protocols [ 26].
Evidence for recombination among L. hochstetteri DAB alleles was detected using single breakpoint (SBP) analysis using small sample AIC (AICC) [ 49].
Mutations in TP53 (exons 4 8), CDKN2A (Cyclin-Dependent Kinase inhibitor 2A, exons 1 α, 2, and 3), and FGFR3 (exons 7, 10, 15) were detected using single strand conformational polymorphism (SSCP) analysis and Sanger sequencing, as detailed 15– 15.
For extended lactation of useful yield, neither of the two QTL were detected using single-QTL methods, while for extended lactation of somatic cells, one of the QTL positions was identified in both single-QTL approaches.
Identical genes for resistance to NCLB were detected using single-marker and haplotype-based associations, as well as A-D test.
No islet hormone mRNA contamination was detected using single-cell nested RT-PCR on RNA from sorted GFP+ splenocytes of chicken beta-actin promoter-driven enhanced GFP (C57BL/6-Tg[ACTB-EGFP]1Osb/J C57BL/6-Tg[ACTB-EGFP]1Osb/J C57BL/6-Tg[ACTB-EGFP]1Osb/Jom wild-transgenicL/6J mice (ESM, Resulthat
The number of these family-specific QTL intervals ranged from 13 (TL) to 36 (LL) across families, and the mean number of families in which a QTL interval was detected using single-family analysis ranged from 1.3 (CL) to 3.1 (SLB).
PCR conditions included an initial denaturing step at 92°C for 2 min, 45 cycles of 92°C for 15 s, 52°C for 15 s, and 68°C for 30 s, and a final extension step at 68°C for 5 min. Mitochondrial DNA variation was detected using single-strand conformation polymorphism (SSCP) [ 61].
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