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In the second part of the study, mice were divided into three groups and then injected intravenously with combinations of VHHs conjugated to different NIR fluorophores to study whether the expression status of HER2 and CAIX could be detected simultaneously in vivo (50 μg of each VHH-IR, thus 100 μg in total): 11A4-680 and B9-800 (n = 8), 11A4-800 and R2-700 (n = 8) or B9-800 and R2-700 (n = 7).
Real-time PCR, however, encounters technical difficulties when multiple mutations from one sample need to be detected simultaneously in a single tube.
Therefore, all four possible sequence variants can be detected simultaneously in one reaction.
Employing chromogenic ISH, up to three different mRNAs can be detected simultaneously in Drosophila embryos [ 3, 4].
Our current and previous studies reveal that RasV12 mutation and Aurora-A overexpression can be detected simultaneously in human bladder and colon cancers.
Apart from the quantitative character, another advantage of the technology is the handling of large sample sets which protein abundance can be detected simultaneously in a high throughput fashion.
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The pathogens were detected simultaneously in 10-fold serial dilutions of TNAs from infected plant material into TNAs of healthy plant up to dilutions 10−5 for viroids and 10−4 for phytoplasmas.
Mitral cells and interneurons were distinguished by the expression of the mitral cell marker, HuC-YC [32], [33], that was detected simultaneously in a separate emission channel.
Thirteen of the 24 patients had antibodies to both nRNP 70 K and nRNP A that were detected simultaneously, in the same serum sample.
Moreover, this analysis also excluded genes that were detected simultaneously in the 3 comparisons (A and B and C), because these are not uniquely related to the influence of sucrose and starch in combination.
Two plasmid genes of EAEC namely aat and aggR were detected simultaneously in assay n5.
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