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This can be detected by adding scavengers in the solution and will be studied in our further experiment.
Both the chromate and dichromate anions are strong oxidizing reagents at low pH: : + 14 + 6 e− → 2 + 21 (ε0 = 1.33 V) They are, however, only moderately oxidizing at high pH: : + 4 + 3 e− → + 5 (ε0 = −0.13 V) Chromium VI) compounds in solution can be detected by adding an acidic hydrogen peroxide solution.
Although such aspects cannot be captured neither by the static networks analyzed in the work nor by the centrality measures tested here, it is possible that some missing essential genes may still be detected by adding missing genetic relations in metabolic networks, if any.
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After a final wash, the membrane signals were detected by adding chemiluminescence detection reagent provided with the kit and the membrane was exposed to X-ray film for different time intervals.
The horseradish peroxidase-conjugated secondary antibody (Pierce Chemical) was used and the signals were detected by adding the enhanced chemiluminescence western blotting detection reagents (Amersham Biosciences, Piscataway, NJ, USA).
Islanding is detected by adding the WSE of each phase to obtain WSE index (WSEI).
These chemical compounds had ironically been detected by adding water brought all the way from Earth.
The surviving cells were detected by adding 20 μL MTT (5 mg/mL) and incubating for 4 h.
The bound phages were detected by adding 50 μl HRP-conjugated anti-M13 antibody (Pharmacia, diluted 5000 times with PBS) followed by an incubation of 1 h.
Bound antibodies were detected by adding rabbit anti-human IgG peroxidase conjugate for 30 min, followed by staining with tetramethylbenzidine for 15 min.
After rinsing with Ca2+ buffer, the binding between AnnV-Bt and the target was detected by adding the ABC Standard Peroxidase Staining Kit (Thermo Fisher Scientific) (200 μL) for more than (over) one hour at room temperature.
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