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Adherent cells have to be detached into a single cell suspension prior to fixation.
However, since flow cytometry is applicable only to suspended cells, adherent cells have to be detached into a single cell suspension prior to the analysis, which possibly perturbs the signaling activity of interest.
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However, these methods require cells to be detached into suspension prior to analysis, an event which could activate many signaling pathways and perturb the biochemical process to be studied.
The image was detached into non-overlapping 8×8 patches.
Then, each low-resolution frame I ′ is detached into N non-overlapping patches {P i |i=1,2,⋯,N} (see Fig. 2).
Briefly, mitotic cells were detached into the media by gentle agitation of the plate and the remaining adherent cells were collected separately after trypsinization.
Cells were treated with 20 ng ml−1 colcemid for 4 h and mitotic cells were detached into the medium by tapping the flask.
It can indeed be detached, with a sharp tug.
As the atmosphere contracted, it would have to rotate faster (to conserve angular momentum), and when centrifugal force exceeded gravity at the outside, a ring of material would be detached, later to condense into a planet.
The legs should be detached and divided into two pieces each and the breast area cut neatly into four sections, once across and once lengthways.
The nanosheets attached to the facetted nanowires could easily be detached from the substrate and dispersed into an aqueous solution via sonication for several seconds, which enabled us to easily prepare TEM samples.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com