The tribes of the west paid little heed to the khans who appeared in dizzying succession in Sarai; the northern Russian princes fell to quarreling and to maneuvering for their own advantage in the internecine politics of the Golden Horde; the Volga Bulgar region was detached by a dissident Tatar prince; and the lands of the east were drawn into the orbit of the Turkic conqueror Timur (Tamerlane).
The super hardness in nano-composite thin films is obtained when the small crystallites are detached by a discrepant boundary with the high cohesive strength (Patscheider et al).
After the J774A.1 cells reached 80% confluency, the cells were detached by a scraper and seeded onto a 6-well plate at a density of 2 × 104 cells per well and incubated overnight.
Margarito, 26, is coming off an impressive TKO victory over Sebastian Lujan in February that ended when Lujan's left ear was detached by a punch.
Other than fragmentation carried out for the purpose of reproduction, the division of the body may happen inadvertently due to part being detached by a predator, or part may be actively shed by the starfish in an escape response.
Next, cell medium was aspirated and cells were detached by a 20 minute incubation with cell dissociation buffer (Invitrogen).
For fluorescence activated cell sorter (FACS), B10 cells were detached by a brief trypsin treatment and stained sequentially with immunofluorescence conjugated antibodies, fixed with 2% paraformaldehyde for 5 min, and then analyzed with flow cytometer (FACS Vantage, Becton Dickinson).
Tumour cells were detached by a nonenzymatic cell dissociation solution and washed twice in cold PBS.
Briefly, the cells were detached by a brief trypsinization, isolated by centrifugation, washed in PBS, resuspended in the Cytosol Extraction Buffer containing a mix of DTT and protease inhibitors, homogenized in an ice-cold tissue grinderm and centrifuged at 700 × g for 10 min at 4°C.
The reaction was stopped by adding 3 ml of FCS containing medium and the epidermal cells could subsequently be detached by gentle scraping with a spattle.
