Two electrodes were designed, using either PVC or Nafion, to immobilise the macrocyclic receptor on a glassy carbon electrode (GCE) surface and used for dopamine (Dop) determination.
Primer pairs for each gene selected were designed using either Japanese quail (Coturnix japonica) or chicken (Gallus gallus) sequence information from the National Center for Biotechnology Information (NCBI, http://www.ncbi.nlm.nih.gov) GenBank database.
From here, new layouts have then been designed using either a genetic algorithm or a particle swarm optimizer.
Molecular fingerprints counting atom pairs at increasing through-space distance intervals were designed using either all atoms (16-bit 3DAPfp) or different atom categories (80-bit 3DXfp).
These primers were designed using either an alignment between the FST and MAGI or were designed with the FST alone.
Unless specified otherwise, primers were designed using either OligoPerfect (Invitrogen Life Technologies), MWG Operon (Eurofins Scientific, Luxembourg) or NCBI Primer Blast NCBI Resource Coordinatorss, 2015) tools.
CDSSs are designed using either rule-based systems that represent knowledge in IF…THEN rules, or machine learning models where mathematical functions estimate risks given patient observations [ 10].
Specific primers and probes for 14 of the 74 selected differentially expressed genes (SCO6808, SCO7657, SCO4295, SCO5013, SCO3088, SCO3285, SCO3286, SCO5166, SCO6958, SCO6638, SCO4648, SCO4702, SCO4705, SCO4717) were designed using either Primer Express v.2 software (Applied Biosystems) or Primer 3 software [ 52].
