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SSRs designed from EST sequences are especially valuable owing to their genome location, which implies constraints on length, motif, abundance and flanking regions, the latter of particular interest in this context, because common primers can be designed to conserved flanking regions.
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The results of our simulations suggest that conservation programs, for wild populations, need to be designed to conserve habitat capable of supporting approximately 7000 adult vertebrates in order to ensure long-term persistence.
The NAE embraces the principle of sustainability in its belief that our uses of the Earth must be designed to conserve and renew the Earth rather than to deplete or destroy it.
Primers were designed to conserved regions of F3H and used to amplify an approximately 350 bp segment for quantitation by PhosphorImaging.
To test whether integrons occur in environments with no known history of antibiotic exposure, PCR primers were designed to conserved regions of the integrase gene and the gene cassette recombination site.
Primers were designed to conserved regions when possible.
Homologous B. oleracea regions were aligned to A. thaliana, and PCR primers were designed to conserved regions, using PrimerQuest (Integrated DNA Technologies).
Conilon using primer pair 18244, which was designed to conserved RBCS1 cDNA regions of the two species.
A series of degenerate primers were designed to conserved regions specific to each of the five vertebrate retinal opsin families (rh1, rh2, lws, sws1 and sws2).
Primers were designed to conserved regions of the rpoD gene by aligning σ factors from Cyanidium caldarium SigC accession number BAA25788), Guillardia theta (BAB87262) and Thalassiosira pseudonana [ 43].
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