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Exact(6)
However, the flanking sequences adjacent to the known sequences cannot be amplified by conventional PCR because the sequences are unknown; therefore, specific primers pairs cannot be designed to amplify such regions.
After the sliding window process is performed, the forward and reverse primers within the overlapping and conserved regions can easily to be designed to amplify the region between them.
When using the family characteristics family- specific primers could be designed to amplify members from specific homologous families with high averages of predicted solubility in E. coli.
Alternatively, the PCR primers can be designed to amplify target cDNA sequences that correspond specifically to a single strand RNA of the pair; i.e., not part of the overlapping sense and antisense RNA strands.
Since formalin-fixed paraffin embedded materials often yield poorly amplifiable DNA, the efficacy of the primer pair is inversely correlated with the length of the amplimers and the primers should be designed to amplify a relatively short PCR fragment [ 29].
1, [ 26, 27] Once these polymorphisms are located, flanking primers can then be designed to amplify these variable length regions thus allowing differentiation of copy numbers using the size of the resultant amplicon.
Similar(54)
A cityscape that makes your heart race and a restaurant that is designed to amplify the thrill.
Amplifiers are designed to amplify signals.
Gene-specific primers were designed to amplify the Exp2 gene.
Fifteen primers were designed to amplify specific altered genes.
Specific primers were designed to amplify individual isoform transcripts.
More suggestions(15)
be intended to amplify
be designed to reinforce
be designed to extend
be designed to enhance
be performed to amplify
be designed to implement
be designed to protect
be designed to accommodate
be designed to raise
be designed to achieve
be designed to order
be designed to carry
be designed to maximize
be designed to promote
be designed to influence
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