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Thus, filters can be designed targeting the two kinds of noises.
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Species-specific probes were designed targeting the 16S 23S intergenic spacer region (ISR) or the 23S rRNA gene.
In addition, a semi-nested polymerase chain reaction (PCR) approach was designed targeting the 16S ribosomal RNA gene.
GA21 specific primers and probe were designed targeting the vector promoter junction region and amplifying a 72-bp DNA fragment.
Sequences were piled up from the most conserved regions of these geographically diverse isolates and TaqMan® primers and probes were designed, targeting the regions with 100% sequence identity.
Two independent primer/probe sets were designed targeting the 18S gene of the ribosomal cistron for the trichodorid species, Paratrichodorus pachydermus and Trichodorus similis.
Species-specific DPO primers were designed targeting the mdh, vvhA, colH and toxR genes for the discrimination of V. cholerae, V. vulnificus, V. alginolyticus and V. parahaemolyticus, respectively.
A new primer set has been designed targeting the 18S rRNA gene for the detection of Plasmodium falciparum in whole blood samples.
C. polykrikoides- and G. impudicum-specific real-time PCR probes were designed targeting the internal transcribed spacer 2 ribosomal DNA region.
Degenerate primers for HoxD-11 were designed targeting the conserved 5' region of exon one and part of the homeo box.
From the SSR-containing EST sequences, primer pairs were designed targeting the SSRs except hexa-nucleotide repeats using Primer3 [ 52].
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