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The primers could not be designed for remaining 7048 (24.9%) microsatellites due to constraint of obtaining enough flanking sequences from either side of identified microsatellites.
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Primer pairs could not be designed for the remaining SSRs, mainly due to difficulties in obtaining sufficient flanking sequences from either side of the identified microsatellites.
The primer pairs could not be designed for the remaining microsatellites, mostly due to the constraint of obtaining sufficient flanking sequences from either side of the identified microsatellites.
Primers could not be designed for the remaining 894 SSR loci because their flanking sequences were either too short or the nature of sequence did not fulfill the criteria for primer design using BatchPrimer3 v 1.0 software [ 26, 27].
No non cross-hybridizing probes could be designed for the 143 remaining CDS (2.5% of the total).
Most human beings will remain unlovely beings to the end, and governmental structures must be designed for such beings.
However, there is also fear that novel pharmacogenomic drugs will remain too costly, be designed for the needs of the wealthy nations, and so constitute an unnecessary "luxury" for most populations.
For CAPS marker analysis, PCR primers were designed for the remaining 34 isotigs from which 21 sequences were successfully amplified in the parental lines ARG1575-2 and HA342.
Primers for HRM analysis were designed for the remaining 17 contigs (including the three contigs that failed in the dCAPS analysis) to obtain PCR products of ∼100 bp.
Based on gained sequence information, specific primers were designed for screening of the remaining secondary positives.
Subsequently, primers were designed for the coding regions of the remaining 13 genes.
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