Sentence examples for be denatured in solution from inspiring English sources

In vivo biotinylated Crf2 is not expected to be denatured in solution.

Clearly, if a native protein was denatured at the electrode surface, its EC responses should not greatly differ from that of the protein, which was denatured in solution and adsorbed in its denatured form at the electrode surface.

The amplified products were denatured in denaturation solution for 10 minutes and then immobilized to a microtiter plate, DNA-BIND 1 × 8 strip well plates, as described in the manufacturer's instructions.

The resulting labeled cDNAs were denatured in hybridization solution (3×SSC, 0.2% SDS, 5×Denhardt's solution and 25% formamide) at 95°C for 3 min before hybridization.

The gel was denatured in a solution of 0.05 M NaOH and 1.5 M NaCl for 30 min, neutralized in a solution of 0.5 M Tris-HCl, pH 7.4, 1.5 M NaCl, and then equilibrated in 20×SSC.

Cellular DNA was denatured in alkaline solution (300 mM NaOH and 200 mM EDTA) at room temperature in the dark for 20 min and then electrophoresed at 25 V and 300 mA for 10 min.

The DNA was denatured in aqueous solution containing desferal and enzymatically hydrolysed.

Each genomic library was denatured in a solution containing excess blocking oligos and Cot-1 DNA.

Chromosomes were denatured in a solution of 70% formamide in 2× SSC at 70°C for 2 min. The denatured probe was placed on the slide and in situ hybridization was carried out overnight in a moist chamber at 37°C.

Chromosomes were denatured in a solution of 70% formamide in 2X SSC at 70° for 2 min, dehydrated in an ethanol series (70%, 90%, and 100%), and air-dried.

Slide-bound chromosomal DNA was denatured in a solution of 70% formamide in 2 × SSC for 6 min at 75°C on preheated slides for 2 hrs at 60°C and dehydrated in a 4°C ethanol series (70% and 100% ethanol for 5 min each).