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Exact(7)
Suitable elements from the finite element model of the structure are deleted until all the elements that can be deleted without affecting the structural (or mesh) integrity have been.
Similarly, although much of the cytoplasmic portion of Cad99C can be deleted without affecting microvillus length, the residual 31 intracellular amino acids may contain recognition sites for cytoplasmic adapter molecules or cytoskeletal components [37].
In Escherichia coli, where two EF-Tu-coding genes are present, either of them may be deleted without affecting the viability of the cell.
It has long been known that large N-terminal segments of autotransporter passenger domains can be deleted without affecting the integration of the β domain into the OM or the secretion of the remaining passenger domain fragment (Dautin and Bernstein, 2007).
The HP requirements for Hε binding are also minimal in that the entire RNase H domain, the C-terminal part of the RT domain, the majority of the spacer and the N-terminal part of TP can be deleted without affecting HP Hε binding.
In the study by Lim et al. [ 18] it was shown that 50 N-terminal amino acids of RPS3A are essential to interact with and to increase the solubility of the HBx (hepatitis B protein X) in hepatocellular carcinoma cells, whereas from the C-terminus up to 101 amino acids could be deleted without affecting this chaperone function.
Similar(53)
In U. maydis, a glycine-rich domain of 37 aa at the C-terminus was deleted without affecting biological activity, however, cysteine residues are necessary for secretion of Pep1 [ 26].
Deletion mapping demonstrated that bases 325-400 of the gag gene sequence present in our original vector could be deleted without significantly affecting virus titre (data not shown).
Most strikingly, of all genes encoding FKBPs and CYPs, a total of 12 can be deleted without seriously affecting the viability of Saccharomyces cerevisiae [ 11].
LANE: You say that nothing in that picture could be withdrawn, could be deleted, without materially damaging the picture?
Finally, Keller draws attention to functional redundancy, whereby seemingly important genes can be deleted without any apparent repercussions.
Related(20)
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be maximized without affecting
be consumed without affecting
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be rotated without affecting
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be deleted without abrogating
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com