Exact(6)
Observations with missing data will be deleted using listwise deletion at either the MDM creation stage or when the analysis is run.
Single FoVs can only be deleted using the ObsVis window, not in ds9.
For example, it affords sufficient time for genes to be deleted using the Cre-LoxP system, as illustrated in Fig 6.
An alternative method is to amplify the whole region including the flanking regions and the gene(s) to be deleted using long-range PCR.
Regulatory elements harboring Candidate Functional SNPs can be deleted using zinc finger-based nucleases (ZFNs), TALE-based nucleases (TALENs), or CRISPR-associated protein 9 (Cas9 -based nuCas9 -basedISPR/Cas9) [ 90, 91].
If not needed, the secondary content be deleted using clear content option or the tab itself can be deleted from the pane using delete tab option.
Similar(54)
sipD gene was deleted using one-step deletion strategy [59].
Genes were deleted using a markerless gene-deletion system as described previously (Sun et al. 2013).
In addition, items that still misfitted after the collapsing, were deleted using a step-wise top-down deletion strategy until the remaining items fit the model [ 83].
To construct a single-deletion "starter" strain library, the TF-encoding open reading frames were deleted using a PCR-based gene replacement strategy conferring uracil prototrophy or kanamycin resistance.
For ExonA-Rev (without CMV or SV40 promoters), the CMV promoter was removed from ExonA-Rev using BglII and BamHI digestion followed by blunt-end ligation, and the SV40 promoter was deleted using DraIII and BstZ17I digestion and ligation.
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