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Because according to the software instruction, specific scale should be defined for the software.
Similarly, x can be defined for introns.
Measurement goals can also be defined from software goals.
Moreover optimization was done using response optimizer part of Minitab software and the values of target and allowable minimum responses were defined for the software and finally it calculated the maximum achievable values for both of the responses and also corresponding coded values of mixture components.
In this step, the specific scale (which was 2 cm in current study) was defined for the software and, based on the defined scale, the area of white and black parts was automatically calculated by software.
The change in expression (ratio of day 56 measurements to day 1 for each patient as calculated by Affymetrix software) was defined for each transcript; these are referred to as fold change (FC) values.
Gene-specific primers were defined for each transcript with the Primer3 software.
Gene-specific primers and the most appropriate universal probe were defined for each transcritpt with the ProbeFinder software [ 50].
Gene-specific primers were defined for each transcritpt with the Primer 3 v.0.4.0 software.
Mortality rates were defined for each class.
NRY lineages were defined for each haplogroup.
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