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Remodelling the badland features creates longer slopes at lower angles, which can be cultivated using conventional farm machinery.
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These populations were cultivated using conventional methods at the Experimental Farm of Seoul National University.
NHEKs and HeLa-229 cells were cultivated using conventional polystyrene dishes or special gas-permeable dishes (Lumox; Greiner Bio-One, Frickenhausen, Germany) with a hydrophilic tissue culture treated bottom membrane as described [46].
mESCs, FSPE fibroblasts, and NSCs were cultivated using established methods.
Hydrocarbon-degrading bacteria Bacillus subtilis N3-1P was cultivated using different DHs of hydrolysate.
E. coli DH10B (Invitrogen) was cultivated using Luria Bertani broth or agar at 37°C.
Escherichia coli strain DH5α (Bethesda Research Laboratories, Gaithersburg, USA) was cultivated using standard techniques [ 21].
Like several other root and tuber crops, yam is cultivated using clonal propagation.
The isolates were cultivated using liquid and solid media as described by [ 4].
The localisation of regions where Bt-maize can be cultivated without impairing conventional maize fields or nature reserves is a challenging task.
Henbane is thoroughly poisonous, but used to be cultivated for medicinal use.
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