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Sub-NIL 08GH3999 could be crossed with selected sub-NILs containing late blight disease resistance QTL from chromosome 5 to combine resistance QTL from two chromosomes (Haggard et al. 2013; Johnson et al. 2012) and potentially increase the level of quantitative resistance to P. infestans.
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53 confirmed F1 plants from the cross of MRQ74/IR 84984-83-15-18-B were croselectedh 14 selected F1(2) plants with two qDTYs (qDTY 2.2 + qDTY 12.1 ) to produce a large number of F1(3) seeds with all three qDTYs (qDTY 2.2, qDTY 3.1, and qDTY 12.1 ).
These S1 2 families were recombined using the bulk entry method, whereby each selected progeny is crossed with each other selected progeny and each cross contributes equally to the next cycle of WQS.
40 virgin female UAS-CsChrimson flies were crossed with 20 males of the selected Gal4 line.
Six (from the eight) experimental lines were chosen based on the success of the crosses, and eight males per line (from the ten that were crossed with a LG1 female) were selected in both the control and the coevolved treatment.
The selected mice were crossed with B6 to obtain pairs of progeny that carried the recombined chromosomal segment.
The outward sensitivity to the treatment was increased fairly quickly despite the fact that selected individuals were crossed with field-caught individuals every generation.
F1 males were crossed with F1 hermaphrodites, and 50 F2 hermaphrodites were selected from each lineage (N2 or LSJ1 males in the P0 generation) to give a total of 100 recombinant inbred lines.
First germplasm is screened for resistance to P. infestans isolates, and selected resistant genotypes are crossed with susceptible genotypes.
The selected haploid segregant was crossed with IMK439 or IMK440 (depending on its mating type) and a new sporulation and spore isolation was performed.
unc-13 or unc-31 mutants were crossed with JPS316 males and F2 worms were selected that exhibited GCaMP3 fluorescence and an uncoordinated phenotype to generate strains JPS496 and JPS495 respectively.
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