At least 50 individual microspheres were counted for each cytokine, and the median fluorescence intensity was used for subsequent calculations.
In subsequent studies of formalin-evoked NK1-R internalization, representative confocal images from L4 L5 were counted for NK1-R expression and NK1-R internalization.
First and subsequent episodes in the same person were counted separately, except for PcP, because patients with PcP received secondary prophylaxis and exited the study.
The RBC-free bone-marrow-derived primary cells were counted using a hemocytometer and adjusted for use in subsequent experiments.
After each period the total number of eggs and subsequent larvae were counted.
For readmission incidents within 30 days during 1 year, the first subsequent readmission was counted.
Cells were counted and volume adjusted to 1 × 10 viable cells/mL for subsequent ELISPOT assay and multimer staining.
Plasma samples were stored at −80°C, and PBMC were counted, frozen at −80°C, and stored in liquid N 2 for subsequent batch analysis.
For these reasons, some first and subsequent event types may not have been counted, resulting in a lower incidence than for first skeletal events (see Table 1).
The number of invasive cells was counted from five random fields from each well and total cells counted for each well were calculated for subsequent statistical analysis.
