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All 300 samples were found to be correctly inserted, and a BLAST search of each sequence against the S. aureus Mu50 genome (NCBI Refseq: NC_002758) confirmed the identity of each ORF.
Ninety percent of the positive single clones were found to be correctly inserted, and a BLAST search of each sequence against the Cpn CWL029 genome (NCBI Refseq: NC_000922.1) confirmed the identity of each ORF.
For instance, we have seen that there is no homologous 3-way junction in our database that can be correctly inserted in 3EGZ (riboswitch in H. sapiens) and 2OIU (synthetic ribozyme).
Similar(57)
Hence, the recovery of correct phylogenetic information from gaps may simply indicate that an accurate guide-tree was used and not necessarily that gaps are correctly inserted following patterns of real past events of insertions and deletions.
The sequencing data revealed that the synthesized DNA fragment was correctly inserted into the vector.
DNA sequence analysis of the five recombinant expression vectors pPICZα-HSA-UTI demonstrated that cDNA encoding human bikunin and human serum albumin (HSA) gene were correctly inserted into pPICZαA vector.
As long as the tube was correctly inserted, the biggest health concerns Irom likely faces now are atrophy in her jaw muscles which will fatigue rapidly from chewing until they build back up—and damage to her nasal cartilage from holding up the tube, which also increases her long-term risk of upper respiratory infections.
It would be Ascruelongony if Irom's mentas or physical healthedetubeoriated after a 16-year force-feeding regimen, thanks to a newasegimen of isolation administered by her old allies.
DNA sequencing was conducted to assure that the AR was correctly inserted in the vector.
PCR products were thus sequenced to ascertain that the targeted sequence was correctly inserted in the genomic DNA.
The resulting colonies were verified using PCR to confirm that the 3×flag-kan fragment was correctly inserted into the chromosome.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com