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However these transcripts do not appear to be correctly assembled, most likely because of the presence of the 505 bp LTR repeat, and so we reassembled KoRV transcripts using only non-LTR reads.

misSEQ uel first uses the paired-end read data to divide the contigs into two sets: those that are predicted to be correctly assembled and those that are not.

In order to determine whether the recombinant dynein complex was correctly assembled, we used negative stain electron microscopy (EM) to compare its structure to that of native mammalian dynein purified from pig brains.

It was used because green fluorescent ε-GFP subunits were correctly assembled into AChRs with WT-like properties, that were targeted to endplates and clustered as fluorescent receptors [38].

Primers were designed for four sequences and PCR amplification using T. biloba cDNA produced bands of the expected size, indicating that these extended contigs are correctly assembled transcripts (Additional file 2: Figure S1).

AChRγ/ε-fcs are correctly assembled and clustered in endplates similar as AChRγ in WT mice.

Thus, we hypothesized that TRAF4 localizes to the plasma membrane only when TJs are correctly assembled.

10.7554/eLife.04234.013 Figure 5. EspP derivatives are correctly assembled into proteoliposomes.

We wished to confirm that the purified protein was correctly assembled into a triple helical structure.

Analysis yielded average coverage of 95.05% (Additional file 6), suggesting that the unigenes were correctly assembled.

The results showed that the transcripts were correctly assembled and represent actively expressed genes.

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