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By using one specific primer and one reverse primer during PCR, the precise sequence of the end of the miRNA opposite to the specific primer could be correctly amplified and validated.
All genes were correctly amplified whatever the species, and led to a single specific product amplification at the expected Tm.
All genes were correctly amplified whatever the species and led to single specific product amplification at the expected Tm.
The resulting DNAs obtained are amplified using linker-dependent PCR.
The complete cytb was amplified using Thermocycler GeneAmp 9700, USA.
Asx exons were amplified using genomic PCR.
PCR products were amplified using AmpliTaqGold360Mastermix Applied Biosystemss).
Env was amplified using a nested PCR.
The product was amplified using TaqMan Universal PCR Master Mix Applied Biosystemss).
Target genes were amplified using specific primers.
cDNA was amplified using standard PCR conditions.
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