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A bioreactor culture could be continuously diluted to optimize growth and avoid over-densification (see also Fouchard et al., 2009).
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Since the cells are continuously diluted, and dilution rate remains fixed throughout the experiment, any change in growth rate will be reflected practically immediate by a change in the optical density.
The p16-positive cells do not cycle and are continuously diluted as the cultures proliferate, while new p16-positive cells are generated in the cultures from proliferating cells, thus maintaining a steady-state frequency of p16-positive cells of approximately 20%.
DNA was subsequently diluted to 8 nM.
Cultures were grown semi-continuously; after each daily sampling, N-replete and N-deplete cultures were diluted to 10 cells mL−1 with BG-11 medium containing 0.1 M nitrate or no N, respectively, causing increasing N starvation in N-deplete cultures.
After incubation in DMEM supplemented with 10% bovine serum at 37°C for 24 h, the antineoplastic drugs were diluted to various concentrations and incubated with the cells continuously for 72 h.
Samples were diluted to 10 pmol/µl in the previous buffer and continuously infused into the ESI ion source at a flow rate of 3 µl/min through a Harvard syringe pump (Harvard Apparatus model 11).
Samples were diluted to approximately 200 ppb.
Samples were diluted to 200 ng/µl.
Total RNA was diluted to uniform concentration.
Viruses were diluted to 2 TCID50/µL.
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