The genetic analyses showed that it contained an open reading frame of 993 bp, which was considered to encode B. bovis l-lactate dehydrogenase (BbLDH: E.C. 1.1.1.27) because of the strikingly high amino acid identities of its gene product to the LDHs of Plasmodium falciparum and Toxoplasma gondii.
Various approaches were considered to encode the history matrix H into a set of features that can be used to train classifiers.
After a detailed analysis of sequence alignments, several contigs and/or singletons that were not automatically assembled were considered to encode a same putative protein (Table 3).
Furthermore, two gene clusters detected in each C. ulcerans genome are considered to encode adhesive pilus structures that are covalently anchored to the corynebacterial cell wall and probably mediate the initial adhesion of the pathogen to host tissues (Table 5).
Besides, omtp was considered to encode a catechol- O-methyltransferase (COMT), a protein family which has ever been found to be horizontally transferred from bacteria to eukaryotes before the separation of animals and fungi [ 27, 28].
As mentioned above, all BLAST hits for a given function whose E-values were below the cut-off and that were classified into at least one of the categories described were considered to encode that function.
Contigs were considered to encode GRPs if the translated amino acid sequence contained a glycine content of at least 20% (with three exceptions among the 60 unique contigs, which contained 11 and 17% glycine).
A gene was considered to encode a homologue of an already identified protein if it had the Expect value (E) in blastp of < e-100 with the next Sordariomycetes member.
Interestingly, RNAi of C05D11.7, an ortholog of PNPLA3 that was considered to encode adipose triglyceride lipase (ATGL) in C. elegans[ 51, 52], led to higher fat and larger lipid droplets compared with the control in C. elegans, similar to other reports on humans and rodents [ 83, 84].
To date, 20 different MC-degrading bacteria have been isolated from rivers, lakes, and biological filters, but without simultaneous detection of the mlr gene cluster, which is considered to encode the hydrolytic proteins involved in the initial steps of MC-degradation [ 41].
