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Interestingly, a further four GH18 domains found elsewhere in the tree also lack the glutamate residue (Dm-CHT12, Dm-CHT10, Dappu-CHT3, and Tenebrio molitor CHT1), indicating that these domains also have a function that does not require hydrolysis of chitin, and should be considered for functional studies.
For the comparison between SC and SNC (DEG list 3; 416 genes), we omitted the logFC filter so that a significant number of genes could be considered for functional analyses.
Other alternatives to a supramolecular organization of the respiratory chain can also be considered for functional advantages such as segregation into microdomains as mentioned in the discussion section of our manuscript.
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Only the significant differentially expressed proteins were considered for functional analysis.
The set of TUSs with high differential expression ≥5 were considered for functional annotation using BLASTX analysis as described above.
Genes predicted to contain at least one occurrence of these significant motif instances were considered for functional enrichment analysis using g:Profiler (26).
A polynomial model of order 3 was assumed for analysis and only probes with a fold change in expression higher or lower than 2 fold in at least one time point were considered for functional analysis.
If one gene probe on the microarray chip corresponded to more than one gene (e.g. multiple isoforms of a gene), all the corresponding genes were considered for functional analysis.
In addition, both compounds 8 and 16 exhibited the desired multi-target profile, which could be considered for further functional efficacy assessment, analog modification for the improvement of selectivity towards A1R, A2AR and PDE10A collectively, and evaluation of their potential synergy in modulating cAMP levels.
Our data together with SLE reports [ 23], suggest that haplotypic allele combinations might be considered for further functional studies.
Of these, 51 feature in our H-List and 129 do not, while six could not be considered for lack of sufficient functional data.
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